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The laboratory manual, by Mc Donald, includes all the procedures used for these methods (1). This was completed by taking an inoculating loop and spreading the unknown out on to a Nutrient Agar plate.This was done using the streak plate method, which is described in the lab manual.
From there, each colony was isolated, incubated and grown on separate plates.
After the morphology was observed and recorded, a Gram stain was performed on each, this procedure is found in the laboratory manual.
The bacterium were incubated and grown at 37 degrees for four days.
Upon observation the isolation plate showed growth of two different colonies.
Culture #1 Biochemical Tests & Results DISCUSSION/CONCLUSION: The first culture’s results were found with the help of the unknown chart, handed out by the instructor.
The unknown tube, #125, was isolated in order to grow two separate cultures.
The two microorganisms were identified; one was a Gram-positive bacteria, the other was a Gram-negative bacteria.
A series of biochemical tests were performed based on the results of the Gram stains.
The tables below illustrate the tests used for each unknown microorganism.
The flow charts show how the conclusions were reached.